Integrin alpha E/CD103 Antibody (2G5.1)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65271

Clone 2G5.1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB100-65271
NB100-65271-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2G5.1

Concentration

1.0 mg/ml

View all Integrin alpha E/CD103 Antibodies »

Product Specifications

Immunogen

Human intestinal lymphocytes

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey, Cynomolgus monkey

Specificity

NB100-65271 recognizes the human CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of human intra-epithelial lymphocytes:- 40% of lamina propria T-cells; 30% of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognizes less than 0.1% bone marrow cells and less than 0.7% PBL.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Integrin alpha E/CD103 Antibody (2G5.1)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:50-1:100

Immunoprecipitation

1:10-1:500
Application Notes
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin alpha E/CD103

CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and approx. 20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.

Alternate Names

CD103, HUMINAE, ITGAE

Entrez Gene IDs

3682 (Human)

Gene Symbol

ITGAE

UniProt

P38570 (Human)

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (2G5.1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Integrin alpha E/CD103 Antibody (2G5.1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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