Skip to main content

Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43370

Clone B-Ly7 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP1-43370-0.1mg
Conjugate
Catalog #

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # B-Ly7

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

The immunogen for this antibody was CD103/Integrin alpha E.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free [NBP1-43370]

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free [NBP1-43370]

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) [NBP1-43370] - Analysis using the FITC conjugate of NBP1-43370. Staining of 3-day PHA-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (blue histogram) or Anti-Human CD103 (Integrin alpha E) FITC (purple histogram).
Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free [NBP1-43370]

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free [NBP1-43370]

Flow Cytometry: Integrin alpha E/CD103 Antibody (B-Ly7) [NBP1-43370] - CD103/Integrin alpha E Antibody (B-Ly7) Staining of 3-day PHA-stimulated normal human peripheral blood cells with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (open histogram) or 0.25 ug of Anti-Human CD103 (Integrin alpha E) Purified (filled histogram) followed by Anti-Mouse IgG FITC .

Applications for Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
The B-Ly7 antibody has been tested by flow cytometric analysis of 2-3 day PHA-activated human peripheral blood leukocytes. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha E/CD103

The B-Ly7 monoclonal antibody reacts with human CD103/Integrin alpha E, the alphaE integrin. CD103/Integrin alpha E non-covalently associates with integrin beta7. CD103/Integrin alpha E is expressed mainly on intraepithelial lymphocytes and a small subset of peripheral lymphocytes. CD103/Integrin alpha E is also expressed by hairy cell leukemia (HCL) and by some chronic B cell lymphocytic leukemias. In vitro stimulation of human T cells with mitogens induces upregulation of CD103/Integrin alpha E. Epithelial cell antigen, E-cadherin, binds to CD103/Integrin alpha E and mediates homing of lymphocytes to the intestinal epithelium.

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin alpha E/CD103 Antibody (B-Ly7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...
Loading...