Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97564

Clone Ber-ACT8 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP1-97564
NBP1-97564-25ug
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Conjugate
Catalog #
Alexa Fluor 350
NBP1-97564AF350
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Alexa Fluor 405
NBP1-97564AF405
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Alexa Fluor 488
NBP1-97564AF488
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Alexa Fluor 532
NBP1-97564AF532
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Alexa Fluor 594
NBP1-97564AF594
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Alexa Fluor 647
NBP1-97564AF647
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Alexa Fluor 700
NBP1-97564AF700
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Alexa Fluor 750
NBP1-97564AF750
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Allophycocyanin
NBP1-97564APC
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Allophycocyanin/Cy7
NBP1-97564APCCY7
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Biotin
NBP1-97564B
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CoraFluor 1
NBP1-97564CL1
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DyLight 350
NBP1-97564UV
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DyLight 405
NBP1-97564V
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DyLight 488
NBP1-97564G
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DyLight 550
NBP1-97564R
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DyLight 594
NBP1-97564DL594
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DyLight 650
NBP1-97564C
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DyLight 680
NBP1-97564FR
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DyLight 755
NBP1-97564IR
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FITC
NBP1-97568
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HRP
NBP1-97564H
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Janelia Fluor 525
NBP1-97564JF525
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Janelia Fluor 549
NBP1-97564JF549
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Janelia Fluor 585
NBP1-97564JF585
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Janelia Fluor 635
NBP1-97564JF635
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Janelia Fluor 646
NBP1-97564JF646
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Janelia Fluor 669
NBP1-97564JF669
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mFluor Violet 450 SE
NBP1-97564MFV450
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mFluor Violet 500 SE
NBP1-97564MFV500
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mFluor Violet 610 SE
NBP1-97564MFV610
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PE
NBP1-97564PE
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PE/Atto594
NBP1-97564PEATT594
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PE/Cy5.5
NBP1-97564PECY55
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PE/Cy7
NBP1-97564PECY7
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PerCP
NBP1-97564PCP
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Key Product Details

Species Reactivity

Human, Primate

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Ber-ACT8

Format

BSA Free

Concentration

1 mg/ml

View all Integrin alpha E/CD103 Antibodies »

Product Specifications

Immunogen

HTLV-1 induced human T cell line MAPS16

Specificity

The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes. HLDA V; WS Code A067

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).
Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on human peripheral blood cells with anti-CD103 (Ber-ACT8) APC.
Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564]

Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.
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Applications for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Integrin alpha E/CD103

CD103 / Integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation

Alternate Names

CD103, HUMINAE, ITGAE

Gene Symbol

ITGAE

Additional Integrin alpha E/CD103 Products

Product Documents for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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