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Integrin beta 1/CD29 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62630

Novus Biologicals, part of Bio-Techne
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NBP2-62630

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Integrin beta 1/CD29 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Integrin beta 1/CD29 Antibody

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62630]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62630]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62630] - Analysis in human prostate and pancreas tissues using NBP2-62630 antibody. Corresponding ITGB1 RNA-seq data are presented for the same tissues.
Western Blot: Integrin beta 1/CD29 Antibody [NBP2-62630]

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-62630]

Western Blot: Integrin beta 1/CD29 Antibody [NBP2-62630] - Analysis in human cell line U-138MG.
Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62630]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62630]

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP2-62630] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Integrin beta 1/CD29 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Integrin beta 1/CD29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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