Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41272
![Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]](https://resources.bio-techne.com/images/products/Integrin-beta-2-CD18-Antibody-M18-2-Low-Endotoxin-Flow-Cell-Surface-NBP1-41272-img0002.jpg "Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # M18/2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
Murine cytotoxic T cell glycoproteins
Specificity
The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2) FITC.![Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]](https://resources.bio-techne.com/images/products/Integrin-beta-2-CD18-Antibody-M18-2-Low-Endotoxin-Flow-Cell-Surface-NBP1-41272-img0001.jpg "Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]")
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]
Flow (Cell Surface): Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin [NBP1-41272] - Surface staining of mouse splenocytes using anti-CD18 antibody (clone M18/2).![Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]](https://resources.bio-techne.com/images/products/nbp1-41272_rat-monoclonal-integrin-beta-2-cd18-antibody-m18-2-low-endotoxin-13120241659351.jpg "Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]")
Flow Cytometry: Integrin beta 2/CD18 Antibody (M18/2) - Low Endotoxin - Azide and BSA Free [NBP1-41272]
Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified antibody (low endotoxin, concentration in sample 16 ug/ml, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in Analysis (surface staining) of murine splenocyte suspension.Applications
Application
Recommended Usage
Flow Cytometry
1 ug/ml
Immunohistochemistry-Frozen
1:200
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Endotoxin Level
Endotoxin level is less than 0.01 EU/ug of the protein
Formulation
PBS (pH 7.4), 0.2 um filter sterilized
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Integrin beta 2/CD18
Alternate Names
CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta
Gene Symbol
ITGB2
Additional Integrin beta 2/CD18 Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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