Integrin beta 2/CD18 Antibody (YFC118.3)

Novus Biologicals, part of Bio-Techne | Catalog # NB200-610

Clone YFC118.3 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NB200-610
NB200-610-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # YFC118.3

Concentration

1 mg/ml

View all Integrin beta 2/CD18 Antibodies »

Product Summary for Integrin beta 2/CD18 Antibody (YFC118.3)

Immunogen

Human neutrophils

Specificity

This recognises the human CD18 cell surface antigen, the integrin beta2 subunit. CD18 is expressed by the majority of leucocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Integrin beta 2/CD18 Antibody (YFC118.3)

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunocytochemistry/ Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunocytochemistry/Immunofluorescence: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - A-D Confocal microscopy of IBA-1 (green staining) immunohistochemistry of RPE flatmounts (RPE autofluorescence visible as orange due to its autofluorescence in the red and green channel) from a healthy donor (A), a geographic atrophy lesion (B), and large drusen (C and D). (A, B, D): orthogonal Z-stack projection; (C): oblique Z-stack projection and dissecting microscope appearance of postmortem large drusen after removal of the overlaying retina (inset). E-G Double-labeling on the subretinal side of the retina (to avoid masking by RPE autofluorescence) of IBA-1+ (E, green fluorescence) and CD18 (F, red fluorescence; G, merge). H. Orthogonal and lateral Z-stack of a subretinal IBA-1+ (green fluorescence) MPs adjacent to the RPE (orange autofluorescence) in the vicinity of a large drusen.
Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Immunohistochemistry-Frozen: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Canine histiocytic sarcoma stained with Integrin beta 2/CD18 Antibody (YFC118.3). IHC-Fr image submitted by a verified customer review.
Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) - BSA Free [NB200-610]

Flow Cytometry: Integrin beta 2/CD18 Antibody (YFC118.3) [NB200-610] - Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD18: FITC.
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Applications for Integrin beta 2/CD18 Antibody (YFC118.3)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000-1:5000

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NB200-610 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 2/CD18

CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.

Alternate Names

CD18, ITGB2, LCAMB, LFA-1, LFA-1 beta, MAC-1 beta, MF17, p150,95 beta

Gene Symbol

ITGB2

UniProt

P05107 (Human)

Additional Integrin beta 2/CD18 Products

Product Documents for Integrin beta 2/CD18 Antibody (YFC118.3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin beta 2/CD18 Antibody (YFC118.3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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