Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ITGB3/1713

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Integrin beta 3/CD61 Antibodies »

Product Summary for Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free

Immunogen

Recombinant human Integrin beta 3/CD61 protein (Uniprot: P05106)

Localization

Cell Surface

Specificity

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61).The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Marker

Platelet Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53393).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free [NBP2-54562]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free [NBP2-54562]

Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (ITGB3/1713) [NBP2-54562] - Formalin-fixed, paraffin-embedded human Spleen stained with CD61 Monoclonal Antibody (ITGB3/1713).

Applications for Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Integrin beta 3/CD61

Platelet membrane glycoprotein Integrin beta-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar membrane glycoprotein complex is present on endothelial cells. Homology suggests that GP IIIa is a member of a family of cell-surface adhesion receptors. (1) Data provide further support for the key role of the cytoplasmic domain of the beta3 integrin in cell adhesion and suggest a potential role for the beta3C integrin subunit in modulating cell-matrix interactions (2). It has been demonstrated that the beta 3 subunit of alpha IIb beta 3 was phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3). be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin (3).

Alternate Names

CD61, GP3A, GPIIIA, INGRB3, ITGB3

Gene Symbol

ITGB3

Additional Integrin beta 3/CD61 Products

Product Documents for Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Integrin beta 3/CD61 Antibody (ITGB3/1713) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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