Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54418
Clone Y2/51 was used by HLDA to establish CD designation.
![Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free [NBP2-54418]](https://resources.bio-techne.com/images/products/Integrin-beta-3-CD61-Antibody-Y2-51-Immunohistochemistry-Paraffin-NBP2-54418-img0001.jpg "Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free [NBP2-54418]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # Y2/51
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free
Immunogen
Phytohemagglutinin (PHA)-stimulated peripheral blood cells
Localization
Cell Surface
Specificity
Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). It associates with the IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61).The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.
Marker
Platelet Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53414).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free [NBP2-54418]
Immunohistochemistry-Paraffin: Integrin beta 3/CD61 Antibody (Y2/51) [NBP2-54418] - Formalin-fixed, paraffin-embedded human Spleen stained with CD61 Monoclonal Antibody (Y2/51).Applications for Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Integrin beta 3/CD61
Alternate Names
CD61, GP3A, GPIIIA, INGRB3, ITGB3
Gene Symbol
ITGB3
Additional Integrin beta 3/CD61 Products
Product Documents for Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free
Product Specific Notices for Integrin beta 3/CD61 Antibody (Y2/51) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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