integrin beta 4 binding protein Antibody (A6A2-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32304
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A6A2-R
Concentration
1 mg/ml
Product Summary for integrin beta 4 binding protein Antibody (A6A2-R)
Immunogen
Recombinant protein within human integrin beta 4 binding protein aa 1-200. (Uniprot: P56537)
Localization
Cytoplasm, Nucleus, nucleolus.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for integrin beta 4 binding protein Antibody (A6A2-R)
Western Blot: integrin beta 4 binding protein Antibody (A6A2-R) [NBP3-32304]
Western Blot: integrin beta 4 binding protein Antibody (A6A2-R) [NBP3-32304] - Western blot analysis of integrin beta 4 binding protein on different lysates with Mouse anti-integrin beta 4 binding protein antibody (NBP3-32304) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat testis tissue lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32304) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Applications for integrin beta 4 binding protein Antibody (A6A2-R)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: integrin beta 4 binding protein
Alternate Names
b(2)gcn, B(2)GCN homolog, B4 integrin interactor, CAB, EIF3Ap27(BBP), eIF-6, eukaryotic translation initiation factor 3A, eukaryotic translation initiation factor 6, ITGB4BPintegrin beta 4 binding protein, p27 beta-4 integrin-binding protein, p27BBP
Gene Symbol
EIF6
Additional integrin beta 4 binding protein Products
Product Documents for integrin beta 4 binding protein Antibody (A6A2-R)
Product Specific Notices for integrin beta 4 binding protein Antibody (A6A2-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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