Integrin beta 4/CD104 Antibody (M126)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23396
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M126
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M126 was generated from a recombinant protein containing amino acid residues in the cytoplasmic region of human Integrin beta 4/CD104. This sequence is found in all three Integrin beta 4/CD104 isoforms and has 90% homology with rat and mouse Integrin beta 4/CD104. [UniProt# P16144]
Specificity
This antibody detects a 200kDa protein corresponding to the molecular mass of Integrin beta 4/CD104 on SDS-PAGE immunoblots of human A431 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Integrin beta 4/CD104 Antibody (M126)
Western Blot: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] -
Western Blot: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-Integrin beta 4/CD104 (Tyr-1526) (lanes 1 & 2) and anti-Integrin beta 4/CD104 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-Integrin beta 4/CD104 (lanes 5 & 6).
Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] -
Immunocytochemistry/Immunofluorescence: Integrin beta 4/CD104 Antibody (M126) [NBP3-23396] - Immunocytochemical labeling of Integrin beta 4/CD104 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-Integrin beta 4/CD104 (Cytoplasmic region) (left) or rabbit polyclonals anti-Integrin beta 4/CD104 (Tyr-1494) (middle) or anti-Integrin beta 4/CD104 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight(R) 594.Applications for Integrin beta 4/CD104 Antibody (M126)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:250
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Integrin beta 4/CD104
Alternate Names
CD104, ITGB4
Gene Symbol
ITGB4
Additional Integrin beta 4/CD104 Products
Product Documents for Integrin beta 4/CD104 Antibody (M126)
Product Specific Notices for Integrin beta 4/CD104 Antibody (M126)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...