Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all INTS1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide derived from a sequence between residues 150 and 200 of human KIAA1440 using the numbering given in entry XP_291222.2 (GeneID 26173).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for INTS1 Antibody

Western Blot: INTS1 Antibody [NB100-509]

Western Blot: INTS1 Antibody [NB100-509]

Western Blot: INTS1 Antibody [NB100-509] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT1 antibodyA300-361A used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: INTS1 Antibody [NB100-509]

Immunoprecipitation: INTS1 Antibody [NB100-509]

Immunoprecipitation: INTS1 Antibody [NB100-509] - Detection of human INTS1 (INT1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INT1 antibody NB100-509 used for IP at 3 ug per reaction. INT1 was also immunoprecipitated by rabbit anti-INT1 antibody from Company B. For blotting immunoprecipitated INT1, NB100-509 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for INTS1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:2500
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: INTS1

INTS1 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).[supplied by OMIM]

Alternate Names

DKFZp586J0619, FLJ46624, integrator complex subunit 1, NET28, UNQ1821/PRO3434

Entrez Gene IDs

26173 (Human)

Gene Symbol

INTS1

UniProt

XP_291222.2 (Human)

Additional INTS1 Products

Product Documents for INTS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for INTS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.