Involucrin Antibody (8H7B3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15461

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15461-100ul
NBP3-15461-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8H7B3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Involucrin Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Involucrin (P07476). KAENPEQQLKQEKTQRDQQLNKQLEEEKKLLDQQLDQELVKRDEQLGMKKEQLLELPEQQEGHLKHLEQQEGQLKHPEQQEGQLELPEQQEGQLELPEQQE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Involucrin Antibody (8H7B3)

Western Blot: Involucrin Antibody (8H7B3) [NBP3-15461]

Western Blot: Involucrin Antibody (8H7B3) [NBP3-15461]

Western Blot: Involucrin Antibody (8H7B3) [NBP3-15461] - Analysis of extracts of Rat lung cells, using Involucrin antibody (NBP3-15461) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461]

Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461]

Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] - Mouse skin using Involucrin Rabbit mAb (NBP3-15461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461]

Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461]

Immunohistochemistry-Paraffin: Involucrin Antibody (8H7B3) [NBP3-15461] - Rat skin using Involucrin Rabbit mAb (NBP3-15461) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for Involucrin Antibody (8H7B3)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Involucrin

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Alternate Names

involucrin

Gene Symbol

IVL

Additional Involucrin Products

Product Documents for Involucrin Antibody (8H7B3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Involucrin Antibody (8H7B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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