Involucrin Antibody (IVRN/827)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44883
![Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/827) [NBP2-44883]](https://resources.bio-techne.com/images/products/Involucrin-Antibody-IVRN-827-Immunohistochemistry-Paraffin-NBP2-44883-img0001.jpg "Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/827) [NBP2-44883]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # IVRN/827
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified involucrin from human keratinocytes (Uniprot: P07476)
Localization
Cytoplasmic
Specificity
It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this monoclonal antibody reacts with a 120kDa protein. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Marker
Squamous Cell Terminal Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11480)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Involucrin Antibody (IVRN/827)
Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/827) [NBP2-44883]
Immunohistochemistry-Paraffin: Involucrin Antibody (IVRN/827) [NBP2-44883] - Human Tonsil stained with Involucrin Monoclonal Antibody (IVRN/827)Applications for Involucrin Antibody (IVRN/827)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.1 - 0.2 ug/ml
Application Notes
Immunofluorescence: 1-2ug/ml. Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37C. Note than enzyme digestion is better than citrate buffer based epitope unmasking.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Involucrin
Additional Involucrin Products
Product Documents for Involucrin Antibody (IVRN/827)
Product Specific Notices for Involucrin Antibody (IVRN/827)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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