Key Product Details

Species Reactivity

Human, Porcine, Canine, Primate

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SY5

Format

BSA Free

Concentration

1 mg/ml

View all Involucrin Antibodies »

Product Specifications

Immunogen

Purified involucrin from human keratinocytes.

Epitope

The epitope maps between codon 421-568 of human involucrin.

Reactivity Notes

There is weak reactivity with Canine, but it does not react with Mouse.

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Involucrin Antibody (SY5) - BSA Free

Western Blot: Involucrin Antibody (SY5)BSA Free [NB100-2727]

Western Blot: Involucrin Antibody (SY5)BSA Free [NB100-2727]

Western Blot: Involucrin Antibody (SY5) [NB100-2727] - Involucrin from primary human epidermal keratinocyte lysates grown in low calcium (0.07 mM) for 5 days or differentiated with high calcium (1.5 mM) for 1-5 days. Block in 5% nonfat dry milk in TBST incubated at RT for 1 hr, antibody 1:500 in TBST, overnight at 4C. Image from verified customer review.
Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) - BSA Free [NB100-2727]

Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) - BSA Free [NB100-2727]

Immunohistochemistry-Paraffin: Involucrin Antibody (SY5) [NB100-2727] - Staining of human Tonsil with Involucrin Monoclonal Antibody (SY5).

Applications for Involucrin Antibody (SY5) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1.0 ug/10^6 cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

0.1-0.2 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Localized to upper spinous and granular layers in normal skin. IHC: Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 degrees Celsius. Note than enzyme digestion is better than citrate buffer based epitope unmasking. This antibody is CyTOF ready. Positive control(s): MCF-7 cells.

Reviewed Applications

Read 1 review rated 4 using NB100-2727 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Involucrin

Stains paraffin embedded human tissue, so will be useful for studies of changes in involucrin expression in pathological studies.

Alternate Names

involucrin

Entrez Gene IDs

3713 (Human); 16447 (Mouse)

Gene Symbol

IVL

Additional Involucrin Products

Product Documents for Involucrin Antibody (SY5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Involucrin Antibody (SY5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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