IQGAP1 Antibody (7I6X1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16879

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16879-100ul
NBP3-16879-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7I6X1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IQGAP1 Antibodies »

Product Summary for IQGAP1 Antibody (7I6X1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IQGAP1 (NP_003861.1). MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IQGAP1 Antibody (7I6X1)

Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879]

Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879]

Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Western blot analysis of extracts of various cell lines, using IQGAP1 Rabbit mAb (NBP3-16879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879]

Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879]

Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Immunohistochemistry of paraffin-embedded human liver using IQGAP1 Rabbit mAb (NBP3-16879) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for IQGAP1 Antibody (7I6X1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IQGAP1

IQGAP1 encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Long Name

IQ Motif Containing GTPase Activating Protein 1

Alternate Names

p195, SAR1

Gene Symbol

IQGAP1

Additional IQGAP1 Products

Product Documents for IQGAP1 Antibody (7I6X1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IQGAP1 Antibody (7I6X1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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