IQGAP1 Antibody (7I6X1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16879
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7I6X1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IQGAP1 (NP_003861.1). MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQ
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for IQGAP1 Antibody (7I6X1)
Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879]
Western Blot: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Western blot analysis of extracts of various cell lines, using IQGAP1 Rabbit mAb (NBP3-16879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879]
Immunohistochemistry-Paraffin: IQGAP1 Antibody (7I6X1) [NBP3-16879] - Immunohistochemistry of paraffin-embedded human liver using IQGAP1 Rabbit mAb (NBP3-16879) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for IQGAP1 Antibody (7I6X1)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: IQGAP1
Long Name
IQ Motif Containing GTPase Activating Protein 1
Alternate Names
p195, SAR1
Gene Symbol
IQGAP1
Additional IQGAP1 Products
Product Documents for IQGAP1 Antibody (7I6X1)
Product Specific Notices for IQGAP1 Antibody (7I6X1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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