IRAK2 Antibody (10G3F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16457

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16457-100ul
NBP3-16457-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10G3F8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IRAK2 Antibodies »

Product Summary for IRAK2 Antibody (10G3F8)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-250 of human IRAK2 (O43187). RVQGVSITRELLWWWGMRQATVQQLVDLLCRLELYRAAQIILNWKPAPEIRCPIPAFPDSVKPEKPLAASVRKAEDEQEEGQPVRMATFPGPGSSPARAHQPAFLQPPEEDAPHSLRSDLPTSSDSKDFSTSIPKQEKLLSLAGDSLFWSEADVVQATDDFNQNRKISQGTFADVYRGHRHGKPFVFKKLRETACSSPGSI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRAK2 Antibody (10G3F8)

Western Blot: IRAK2 Antibody (10G3F8) [NBP3-16457]

Western Blot: IRAK2 Antibody (10G3F8) [NBP3-16457]

Western Blot: IRAK2 Antibody (10G3F8) [NBP3-16457] - Western blot analysis of extracts of various cell lines, using IRAK2 Rabbit mAb (NBP3-16457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: IRAK2 Antibody (10G3F8) [NBP3-16457]

Immunohistochemistry-Paraffin: IRAK2 Antibody (10G3F8) [NBP3-16457]

Immunohistochemistry-Paraffin: IRAK2 Antibody (10G3F8) [NBP3-16457] - Immunohistochemistry of paraffin-embedded rat kidney using IRAK2 Rabbit mAb (NBP3-16457) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for IRAK2 Antibody (10G3F8)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRAK2

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

Long Name

Interleukin-1 Receptor-Associated Kinase 2

Alternate Names

interleukin-1 receptor associated kinase-2, interleukin-1 receptor-associated kinase 2, interleukin-1 receptor-associated kinase-like 2, IRAK-2, MGC150550

Gene Symbol

IRAK2

Additional IRAK2 Products

Product Documents for IRAK2 Antibody (10G3F8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IRAK2 Antibody (10G3F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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