IRF3 [p Ser386] Antibody (4H10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26416

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26416-100ul
NBP3-26416-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4H10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IRF3 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human IRF3 [p Ser386] [UniProt Q14653]

Modification

p Ser 386

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRF3 [p Ser386] Antibody (4H10)

IRF3 [p Ser386] Antibody (4H10)

Western Blot: IRF3 [p Ser386] Antibody (4H10) [NBP3-26416] -

Western Blot: IRF3 [p Ser386] Antibody (4H10) [NBP3-26416] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or not).
All lanes: IRF3 [p Ser386] Antibody at 1.03ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 47 KDa
Observed band size: 47 KDa

Applications for IRF3 [p Ser386] Antibody (4H10)

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: IRF3

IRF3 is a family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3).

Long Name

Interferon Regulatory Factor 3

Alternate Names

interferon regulatory factor 3, IRF-3

Gene Symbol

IRF3

Additional IRF3 Products

Product Documents for IRF3 [p Ser386] Antibody (4H10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IRF3 [p Ser386] Antibody (4H10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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