IRF6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51911

Novus Biologicals, part of Bio-Techne
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NBP1-51911
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Block/Neutralize, Chemotaxis, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all IRF6 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TPSMQLPPALPPQ corresponding to C-Terminus according to NP_006138.1.

Epitope

C-TPSMQLPPALPPQ

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25358290).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for IRF6 Antibody

Western Blot: IRF6 Antibody [NBP1-51911]

Western Blot: IRF6 Antibody [NBP1-51911]

Western Blot: IRF6 Antibody [NBP1-51911] - HEK293 overexpressing IRF6 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911]

Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911]

Immunohistochemistry-Paraffin: IRF6 Antibody [NBP1-51911] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: IRF6 Antibody [NBP1-51911]

Western Blot: IRF6 Antibody [NBP1-51911]

Western Blot: IRF6 Antibody [NBP1-51911] - (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
View 1 more images

Applications for IRF6 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 3 ug/ml

Immunohistochemistry-Paraffin

2 - 3 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.2 - 0.6 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 53.1 kDa band according to NP_006138.1). In transfected HEK293 transiently expressing IRF6 a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows strong nuclear staining in DCT and glomerulus. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 21807998).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRF6

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. [provided by RefSeq]

Long Name

Interferon Regulatory Factor 8

Alternate Names

LPS, OFC6, PIT, PPS, VWS

Entrez Gene IDs

3664 (Human); 54139 (Mouse)

Gene Symbol

IRF6

UniProt

NP_006138.1 (Human)

Additional IRF6 Products

Product Documents for IRF6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRF6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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