Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all IRGC Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC. The immunogen is located within the last 50 amino acids of IRGC. Amino Acid Squence: CLDSWKKHDSEEK

Specificity

Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRGC Antibody - BSA Free

Western Blot: IRGC AntibodyBSA Free [NBP1-77364]

Western Blot: IRGC AntibodyBSA Free [NBP1-77364]

Western Blot: IRGC Antibody [NBP1-77364] - Mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: IRGC Antibody - BSA Free [NBP1-77364]

Immunocytochemistry/ Immunofluorescence: IRGC Antibody - BSA Free [NBP1-77364]

Immunocytochemistry/Immunofluorescence: IRGC Antibody [NBP1-77364] - A20 cells with IRGC antibody at 2.5 ug/ml.
IRGC Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: IRGC Antibody - BSA Free [NBP1-77364] -

Immunocytochemistry/ Immunofluorescence: IRGC Antibody - BSA Free [NBP1-77364] - Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 ug/mL.

Applications for IRGC Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5-20 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IRGC

Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans. Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.

Alternate Names

CINEMA, EC 3.6.5, EC 3.6.5.-, IIGP5, Immunity-related GTPase cinema 1, immunity-related GTPase family, cinema, immunity-related GTPase family, cinema 1, interferon inducible GTPase 5, interferon-inducible GTPase 5, IRGC1, R30953_1

Gene Symbol

IRGC

UniProt

NP_062558 (Human)

Additional IRGC Products

Product Documents for IRGC Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRGC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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