Irisin/FNDC5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14024
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: EDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKEMGRNQQLR
Reactivity Notes
Mouse reactivity reported from a verified customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Irisin/FNDC5 Antibody
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024]
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Specific positive staining can be observed in a subpopulation of mouse gastric mucosa cells. Green is FNDC5. Image from verified customer review.Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024]
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-14024] - Staining of human skeletal muscle shows granular cytoplasmic positivity in myocytes.Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] -
Western Blot: Irisin/FNDC5 Antibody [NBP2-14024] - Comparison of Irisin/FNDC5 expression levels detected by Western blot in Normal Human Keratinocytes (HaCat) and Larynx Epidermoid Carcinoma 2 (HEp-2) (A). The mean value of the densitometric optical analysis of the 3 repeats of Western blot detecting irisin expression levels (B). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35053200/) licensed under a CC-BY license.Applications for Irisin/FNDC5 Antibody
Application
Recommended Usage
Immunofluorescence
Reported in scientific literature (PMID:31614634).
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20-1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 5 using NBP2-14024 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Irisin/FNDC5
Long Name
Fibronectin Type III Domain Containing 5
Alternate Names
FNDC5, FRCP2
Gene Symbol
FNDC5
Additional Irisin/FNDC5 Products
Product Documents for Irisin/FNDC5 Antibody
Product Specific Notices for Irisin/FNDC5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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