Irisin/FNDC5 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59680

Novus Biologicals, part of Bio-Techne
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NBP2-59680
NBP2-59680SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Camel

Cited:

Human, Camel

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Irisin/FNDC5 Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an internal region of the human Irisin/FNDC5 protein (amino acids 32-143) [UniProt Q8NAU1]

Reactivity Notes

Use in Camel reported in scientific literature (PMID:33424357)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Irisin/FNDC5 Antibody - BSA Free

Western Blot: Irisin/FNDC5 AntibodyBSA Free [NBP2-59680]

Western Blot: Irisin/FNDC5 AntibodyBSA Free [NBP2-59680]

Western Blot: Irisin/FNDC5 Antibody [NBP2-59680] - Recombinant human Irisin protein was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Irisin/FNDC5 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody - BSA Free [NBP2-59680]

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody - BSA Free [NBP2-59680]

Immunohistochemistry-Paraffin: Irisin/FNDC5 Antibody [NBP2-59680] - IHC analysis of a formalin fixed and paraffin embedded human kidney tissue section (NBP1-78269) using 1:200 dilution of Irisin/FNDC5 antibody. The signal was developed with HRP-DAB detection and the sections were counterstained with hematoxylin. The antibody generated a membrane cytoplasmic staining in almost all of the cell types in the tested section.

Applications for Irisin/FNDC5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

0.5 - 1.0 ug/ml
Application Notes
Irisin/FNDC5 has a theoretical molecular weight of 23 kDa, however this protein is highly glycosylated and will run higher in WB.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation. Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise. Irisin induces expression of peroxisome proliferator-activated receptor gamma, co-activator PGC1 alpha and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes. A similar function in humans is suggested. Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

Long Name

Fibronectin Type III Domain Containing 5

Alternate Names

FNDC5, FRCP2

Gene Symbol

FNDC5

Additional Irisin/FNDC5 Products

Product Documents for Irisin/FNDC5 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Irisin/FNDC5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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