IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16898

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IRS1 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S636 & S639 of human IRS1 (NP_005535.1). MSPKSV

Modification

p Ser 636, p Ser 639

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

Western Blot: IRS1 [p Ser639, p Ser636] AntibodyAzide and BSA Free [NBP3-16898]

Western Blot: IRS1 [p Ser639, p Ser636] AntibodyAzide and BSA Free [NBP3-16898]

Western Blot: IRS1 [p Ser639, p Ser636] Antibody [NBP3-16898] - Western blot analysis of extracts of 293T cells, using IRS1 antibody (NBP3-16898) at 1:500 dilution.293T cells were treated by IGF-1 (50 ng/ml) at 37C for 5 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IRS1

Insulin receptor substrates (IRS), the major intracellular substrates of the insulin receptor (IR), are adaptor proteins that transduce signals from the IR to downstream effectors that are important for the biological effect of insulin (1-2). After insulin stimulation, IRS proteins are rapidly phosphorylated on multiple tyrosine residues. Once phosphorylated, IRS proteins bind and activate Grb-2, SHP2 and the PI3-K p85 subunit (2-3). IRS-1 functions as one of the key regulators of IR and the insulin-like growth factor-1 receptor. Disruption of IRS-1 causes growth retardation and insulin resistance associated with hypertension, hypertriglyceridemia, and impaired endothelium-dependent vascular relaxation (4-5)

Long Name

Insulin Receptor Substrate 1

Alternate Names

HIRS-1, insulin receptor substrate 1, IRS-1

Gene Symbol

IRS1

Additional IRS1 Products

Product Documents for IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for IRS1 [p Ser639, p Ser636] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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