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IRS4 Antibody (5B9)

Novus Biologicals, part of Bio-Techne | Catalog # H00008471-M01

Novus Biologicals, part of Bio-Techne
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H00008471-M01

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Sandwich ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

IRS4 (NP_003595, 850 a.a. ~ 950 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAE

Specificity

IRS4 - insulin receptor substrate 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for IRS4 Antibody (5B9)

Western Blot: IRS4 Antibody (5B9) [H00008471-M01]

Western Blot: IRS4 Antibody (5B9) [H00008471-M01]

Western Blot: IRS4 Antibody (5B9) [H00008471-M01] - IRS4 monoclonal antibody (M01), clone 5B9 Analysis of IRS4 expression in HeLa.
Sandwich ELISA: IRS4 Antibody (5B9) [H00008471-M01] - Detection limit for recombinant GST tagged IRS4 is 0.03 ng/ml as a capture antibody.

Applications for IRS4 Antibody (5B9)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRS4

IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation..

Alternate Names

insulin receptor substrate 4,160 kDa phosphotyrosine protein, IRS-4pp160, Phosphoprotein of 160 kDa, PY160

Entrez Gene IDs

8471 (Human)

Gene Symbol

IRS4

OMIM

603510 (Human)

Additional IRS4 Products

Product Documents for IRS4 Antibody (5B9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IRS4 Antibody (5B9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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