IRX3 Antibody (2H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00079191-M08

Novus Biologicals, part of Bio-Techne
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H00079191-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all IRX3 Antibodies »

Product Specifications

Immunogen

IRX3 (NP_077312, 182 a.a. ~ 285 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RRRLKKENKMTWAPRSRTDEEGNAYGSEREEEDEEEDEEDCKRELELEEEELGGEEEDTGGEGLADDDEDEEIDLENLDGAATEPELSLAGAARRDGDLGLGPI

Reactivity Notes

Human. Other species not tested.

Specificity

IRX3 - iroquois homeobox protein 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for IRX3 Antibody (2H3)

Western Blot: IRX3 Antibody (2H3) [H00079191-M08]

Western Blot: IRX3 Antibody (2H3) [H00079191-M08]

Western Blot: IRX3 Antibody (2H3) [H00079191-M08] - Analysis of IRX3 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: IRX3 Antibody (2H3) [H00079191-M08]

Immunocytochemistry/ Immunofluorescence: IRX3 Antibody (2H3) [H00079191-M08]

Immunocytochemistry/Immunofluorescence: IRX3 Antibody (2H3) [H00079191-M08] - Analysis of monoclonal antibody to IRX3 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: IRX3 Antibody (2H3) [H00079191-M08]

ELISA: IRX3 Antibody (2H3) [H00079191-M08]

ELISA: IRX3 Antibody (2H3) [H00079191-M08] - Detection limit for recombinant GST tagged IRX3 is approximately 0.03ng/ml as a capture antibody.

Applications for IRX3 Antibody (2H3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: IRX3

IRX3 is a member of the Iroquois homeobox gene family (see IRX1; MIM 606197) and plays a role in an early step ofneural development (Bellefroid et al., 1998 (PubMed 9427753)). Members of this family appear to play multiple rolesduring pattern formation of vertebrate embryos (Lewis et al., 1999 (PubMed 10370142)).(supplied by OMIM)

Alternate Names

Homeodomain protein IRXB1, iroquois homeobox 3, Iroquois homeobox protein 3, iroquois-class homeodomain protein IRX-1, iroquois-class homeodomain protein IRX-3, IRX-1, IRXB1FLJ99187

Entrez Gene IDs

79191 (Human)

Gene Symbol

IRX3

UniProt

NP_077312 (Human)

Additional IRX3 Products

Product Documents for IRX3 Antibody (2H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IRX3 Antibody (2H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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