ISCU Antibody (3B8-1C4)
Novus Biologicals, part of Bio-Techne | Catalog # H00023479-M01
Key Product Details
Species Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3B8-1C4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NIFUN (AAH11906, 26 a.a. ~ 167 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK
Reactivity Notes
Human. Other species not tested.
Specificity
NIFUN - NifU-like N-terminal domain containing
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for ISCU Antibody (3B8-1C4)
![Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01]](https://resources.bio-techne.com/images/products/ISCU-Antibody-3B8-1C4-Western-Blot-H00023479-M01-img0007.jpg "Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01]")
Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01]
Western Blot: ISCU Antibody (3B8-1C4) [H00023479-M01] - NIFUN monoclonal antibody (M01), clone 3B8-1C4 Analysis of NIFUN expression in HL-60.![Immunocytochemistry/ Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01]](https://resources.bio-techne.com/images/products/ISCU-Antibody-3B8-1C4-Immunocytochemistry-Immunofluorescence-H00023479-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01]")
Immunocytochemistry/ Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01]
Immunocytochemistry/Immunofluorescence: ISCU Antibody (3B8-1C4) [H00023479-M01] - Analysis of monoclonal antibody to NIFUN on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: ISCU Antibody (3B8-1C4) [H00023479-M01] - Detection limit for recombinant GST tagged NIFUN is approximately 0.03ng/ml as a capture antibody.
Applications for ISCU Antibody (3B8-1C4)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ISCU
Alternate Names
2310020H20Rik, HML, hnifU, iron-sulfur cluster assembly enzyme ISCU, mitochondrial, iron-sulfur cluster scaffold homolog (E. coli), IscU, IscU iron-sulfur cluster scaffold homolog, IscU iron-sulfur cluster scaffold homolog (E. coli), ISU2, MGC74517, NIFU, NifU-like N-terminal domain containing, NifU-like N-terminal domain-containing protein, NifU-like protein, NIFUN
Entrez Gene IDs
23479 (Human)
Gene Symbol
ISCU
UniProt
Additional ISCU Products
Product Documents for ISCU Antibody (3B8-1C4)
Product Specific Notices for ISCU Antibody (3B8-1C4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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