ISG15 Activating Enzyme/UBE1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00007318-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ISG15 Activating Enzyme/UBE1L Antibodies »

Product Specifications

Immunogen

UBE1L (AAH06378, 1 a.a. - 1012 a.a.) full-length human protein. MDALDASKLLDEELYSRQLYVLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWSDLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQVVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQDPTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVELNDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTALLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVVGLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQEVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIAVFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMDHIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTTEHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGSATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFEELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCVAWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTNQDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFASNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDFVVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYKVVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVPAGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLRVTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

Specificity

UBE1L - ubiquitin-activating enzyme E1-like,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for ISG15 Activating Enzyme/UBE1L Antibody

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P]

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P]

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P] - Analysis of UBE1L expression in human liver.
Immunocytochemistry/ Immunofluorescence: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P]

Immunocytochemistry/ Immunofluorescence: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P]

Immunocytochemistry/Immunofluorescence: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P] - Analysis of purified antibody to UBA7 on HeLa cell. (antibody concentration 10 ug/ml)
Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P]

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P]

Western Blot: ISG15 Activating Enzyme/UBE1L Antibody [H00007318-B01P] - Analysis of UBA7 expression in transfected 293T cell line by UBA7 polyclonal antibody. Lane 1: UBE1L transfected lysate(111.32 KDa). Lane 2: Non-transfected lysate.

Applications for ISG15 Activating Enzyme/UBE1L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and tissue lysate for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ISG15 Activating Enzyme/UBE1L

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq]

Alternate Names

D8, UBA1B, UBA7, UBE2

Entrez Gene IDs

7318 (Human)

Gene Symbol

UBA7

UniProt

AAH06378 (Human)

Additional ISG15 Activating Enzyme/UBE1L Products

Product Documents for ISG15 Activating Enzyme/UBE1L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ISG15 Activating Enzyme/UBE1L Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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