Islet-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98913
![Western Blot: Islet-2 Antibody [NBP2-98913]](https://resources.bio-techne.com/images/products/Islet-2-Antibody-Western-Blot-NBP2-98913-img0002.jpg "Western Blot: Islet-2 Antibody [NBP2-98913]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Islet-2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Islet-2 Antibody
Western Blot: Islet-2 Antibody [NBP2-98913]
Western Blot: Islet-2 Antibody [NBP2-98913] - Anti-Islet-2 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 38 kDa![Immunocytochemistry/ Immunofluorescence: Islet-2 Antibody [NBP2-98913]](https://resources.bio-techne.com/images/products/Islet-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98913-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Islet-2 Antibody [NBP2-98913]")
Immunocytochemistry/ Immunofluorescence: Islet-2 Antibody [NBP2-98913]
Immunocytochemistry/Immunofluorescence: Islet-2 Antibody [NBP2-98913] - Immunofluorescence staining of Islet-2 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Islet-2 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red).Positive staining was localized to nucleus.Applications for Islet-2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1000-1:5000
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Islet-2
Alternate Names
ISL2, Islet2
Gene Symbol
ISL2
Additional Islet-2 Products
Product Documents for Islet-2 Antibody
Product Specific Notices for Islet-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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