Isoleucyl tRNA synthetase Antibody (3D3)
Novus Biologicals, part of Bio-Techne | Catalog # H00003376-M03
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3D3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
IARS (NP_038203.1, 1172 a.a. ~ 1262 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF
Specificity
IARS - isoleucine-tRNA synthetase (3D3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Isoleucyl tRNA synthetase Antibody (3D3)
![Western Blot: Isoleucyl tRNA synthetase Antibody (3D3) [H00003376-M03]](https://resources.bio-techne.com/images/products/Isoleucyl-tRNA-synthetase-Antibody-3D3-Western-Blot-H00003376-M03-img0003.jpg "Western Blot: Isoleucyl tRNA synthetase Antibody (3D3) [H00003376-M03]")
Western Blot: Isoleucyl tRNA synthetase Antibody (3D3) [H00003376-M03]
Western Blot: Isoleucyl tRNA synthetase Antibody (3D3) [H00003376-M03] - Analysis of IARS expression in transfected 293T cell line by IARS monoclonal antibody (M03), clone 3D3. Lane 1: IARS transfected lysatE (120.6 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: Isoleucyl tRNA synthetase Antibody (3D3) [H00003376-M03] - Detection limit for recombinant GST tagged IARS is 0.1 ng/ml as a capture antibody.
Applications for Isoleucyl tRNA synthetase Antibody (3D3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Isoleucyl tRNA synthetase
Alternate Names
EC 6.1.1, EC 6.1.1.5, FLJ20736, IARS1, ILERS, ILRS, IRS, isoleucine tRNA ligase 1, cytoplasmic, Isoleucine--tRNA ligase, isoleucyl-tRNA synthetase, isoleucyl-tRNA synthetase, cytoplasmic, PRO0785
Entrez Gene IDs
3376 (Human)
Gene Symbol
IARS1
UniProt
Additional Isoleucyl tRNA synthetase Products
Product Documents for Isoleucyl tRNA synthetase Antibody (3D3)
Product Specific Notices for Isoleucyl tRNA synthetase Antibody (3D3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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