Isoleucyl tRNA synthetase Antibody (C1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-41682

Novus Biologicals, part of Bio-Techne
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NBP3-41682
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C1

Concentration

1 mg/ml

View all Isoleucyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Human Isoleucyl tRNA synthetase (Uniprot#: P41252; Met1-Arg500 (Uniprot#: P41252)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Isoleucyl tRNA synthetase Antibody (C1)

Isoleucyl tRNA synthetase Antibody (C1)

Western Blot: Isoleucyl tRNA synthetase Antibody (C1) [NBP3-41682] -

Western Blot: Isoleucyl tRNA synthetase Antibody (C1) [NBP3-41682] - Sample: A549 cell lysate Primary Ab: 2ug/ml NBP3-41682; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Applications for Isoleucyl tRNA synthetase Antibody (C1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20ug/mL

Immunohistochemistry

5-20ug/mL

Western Blot

0.01-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Isoleucyl tRNA synthetase

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Two alternatively spliced variants have been isolated that represent alternate 5' UTRs. [provided by RefSeq]

Alternate Names

EC 6.1.1, EC 6.1.1.5, FLJ20736, IARS1, ILERS, ILRS, IRS, isoleucine tRNA ligase 1, cytoplasmic, Isoleucine--tRNA ligase, isoleucyl-tRNA synthetase, isoleucyl-tRNA synthetase, cytoplasmic, PRO0785

Gene Symbol

IARS1

Additional Isoleucyl tRNA synthetase Products

Product Documents for Isoleucyl tRNA synthetase Antibody (C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Isoleucyl tRNA synthetase Antibody (C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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