Isopeptidase T/USP5 Antibody (OTI2E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02648

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI2E3

Concentration

0.45 mg/ml

View all Isopeptidase T/USP5 Antibodies »

Product Summary for Isopeptidase T/USP5 Antibody (OTI2E3)

Immunogen

Full length human recombinant protein of human USP5 (NP_003472) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

93.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isopeptidase T/USP5 Antibody (OTI2E3)

Western Blot: USP5 Antibody (2E3) [NBP2-02648] Analysis of extracts (35ug) from 9 different cell lines by using anti-USP5 monoclonal antibody.
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody (OTI2E3) [NBP2-02648]

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody (OTI2E3) [NBP2-02648]

Immunohistochemistry-Paraffin: USP5 Antibody (2E3) [NBP2-02648] - Staining of paraffin-embedded Human thyroid tissue using anti-USP5 mouse monoclonal antibody.
Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3) [NBP2-02648]

Western Blot: Isopeptidase T/USP5 Antibody (OTI2E3) [NBP2-02648]

Western Blot: USP5 Antibody (2E3) [NBP2-02648] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5.
View 6 more images

Applications for Isopeptidase T/USP5 Antibody (OTI2E3)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.45 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Isopeptidase T/USP5

Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM].

Long Name

Ubiquitin Specific Protease 5

Alternate Names

IsoT, USP5

Entrez Gene IDs

8078 (Human)

Gene Symbol

USP5

Additional Isopeptidase T/USP5 Products

Product Documents for Isopeptidase T/USP5 Antibody (OTI2E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Isopeptidase T/USP5 Antibody (OTI2E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.