Isopeptidase T/USP5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32590

Novus Biologicals, part of Bio-Techne
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NBP1-32590
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (99%), Rat (99%), Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Isopeptidase T/USP5 Antibodies »

Product Summary for Isopeptidase T/USP5 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Isopeptidase T/USP5. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Isopeptidase T/USP5 Antibody

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590]

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590]

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590] - Sample(30 ug whole cell lysate)A:MOLT4 B:Raji 7. 5% SDS PAGE, antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-32590]

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-32590]

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-32590] - Mouse kidney. USP5 antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590]

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590]

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-32590] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE; antibody diluted at 1:1000.
View 1 more images

Applications for Isopeptidase T/USP5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Isopeptidase T/USP5

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Long Name

Ubiquitin Specific Protease 5

Alternate Names

IsoT, USP5

Gene Symbol

USP5

Additional Isopeptidase T/USP5 Products

Product Documents for Isopeptidase T/USP5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Isopeptidase T/USP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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