Isopeptidase T/USP5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83089

Novus Biologicals, part of Bio-Techne
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NBP1-83089
NBP1-83089-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Isopeptidase T/USP5 Antibodies »

Product Summary for Isopeptidase T/USP5 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Isopeptidase T/USP5 Antibody

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-83089]

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-83089]

Western Blot: Isopeptidase T/USP5 Antibody [NBP1-83089] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: Isopeptidase T/USP5 Antibody [NBP1-83089]

Immunocytochemistry/ Immunofluorescence: Isopeptidase T/USP5 Antibody [NBP1-83089]

Immunocytochemistry/Immunofluorescence: Isopeptidase T/USP5 Antibody [NBP1-83089] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-83089]

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-83089]

Immunohistochemistry-Paraffin: Isopeptidase T/USP5 Antibody [NBP1-83089] - Staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
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Applications for Isopeptidase T/USP5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Isopeptidase T/USP5

Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome. Alternate names for USP include ubiquitin carboxyl-terminal hydrolase 5, ubiquitin thioesterase 5, ubiquitin-specific-processing protease 5, deubuiquitinating enzyme 5, isopeptidase T, and ISOT.

Long Name

Ubiquitin Specific Protease 5

Alternate Names

IsoT, USP5

Gene Symbol

USP5

Additional Isopeptidase T/USP5 Products

Product Documents for Isopeptidase T/USP5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Isopeptidase T/USP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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