ITCH/AIP4 Antibody (01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06616

Novus Biologicals, part of Bio-Techne
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NBP3-06616-100ul
NBP3-06616-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 01

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ITCH/AIP4 Antibodies »

Product Specifications

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ITCH/AIP4 (Accession#: NP_113671.3; Arg526-Glu903).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ITCH/AIP4 Antibody (01)

Immunocytochemistry/ Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616]

Immunocytochemistry/ Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616]

Immunocytochemistry/Immunofluorescence: ITCH/AIP4 Antibody (01) [NBP3-06616] - Staining of Human ITCH in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human ITCH monoclonal antibody (1:60) at 37? 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow Cytometry: ITCH/AIP4 Antibody (01) [NBP3-06616]

Flow Cytometry: ITCH/AIP4 Antibody (01) [NBP3-06616]

Flow Cytometry: ITCH/AIP4 Antibody (01) [NBP3-06616] - Analysis of Human ITCH expression on HeLa cells. The cells were treated, stained with purified anti-Human ITCH, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Applications for ITCH/AIP4 Antibody (01)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ITCH/AIP4

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Long Name

Itchy E3 Ubiquitin Protein Ligase

Alternate Names

AIF4, AIP4, NAPP1

Gene Symbol

ITCH

Additional ITCH/AIP4 Products

Product Documents for ITCH/AIP4 Antibody (01)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ITCH/AIP4 Antibody (01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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