ITCH/AIP4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81837

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ITCH/AIP4 Antibodies »

Product Specifications

Immunogen

ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH . The immunogen is located within amino acids 40 - 90 of ITCH. Amino Acid Squence: NNTNSPKWKQPLTV

Specificity

ITCH/AIP4 antibody is human, mouse and rat reactive. At least three isoforms of ITCH/AIP4 are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ITCH/AIP4 Antibody - BSA Free

Western Blot: ITCH/AIP4 AntibodyBSA Free [NBP2-81837]

Western Blot: ITCH/AIP4 AntibodyBSA Free [NBP2-81837]

Western Blot: ITCH/AIP4 Antibody [NBP2-81837] - Western blot analysis of ITCH/AIP4 in 3T3 cell lysate with ITCH/AIP4 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Applications for ITCH/AIP4 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ITCH/AIP4

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

Long Name

Itchy E3 Ubiquitin Protein Ligase

Alternate Names

AIF4, AIP4, NAPP1

Gene Symbol

ITCH

Additional ITCH/AIP4 Products

Product Documents for ITCH/AIP4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITCH/AIP4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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