ITIH2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31750

Novus Biologicals, part of Bio-Techne
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NBP2-31750
NBP2-31750-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ITIH2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPGAKVQFELHYQEVKWRKLGSYEHRIYLQPGRLAKHLEVDVWVIEPQGLRFLHVPDTFEGHFDGVPVISKGQQKAHVSFKPTVAQQRICPNCRET

Reactivity Notes

Mouse reactivity reported in the scientific literature (PMID: 29855321).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ITIH2 Antibody

Immunocytochemistry/ Immunofluorescence: ITIH2 Antibody [NBP2-31750]

Immunocytochemistry/ Immunofluorescence: ITIH2 Antibody [NBP2-31750]

Immunocytochemistry/Immunofluorescence: ITIH2 Antibody [NBP2-31750] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: ITIH2 Antibody [NBP2-31750]

Immunohistochemistry-Paraffin: ITIH2 Antibody [NBP2-31750]

Immunohistochemistry-Paraffin: ITIH2 Antibody [NBP2-31750] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunocytochemistry/ Immunofluorescence: ITIH2 Antibody [NBP2-31750]

Immunocytochemistry/ Immunofluorescence: ITIH2 Antibody [NBP2-31750]

Immunocytochemistry/Immunofluorescence: ITIH2 Antibody [NBP2-31750] - HC-HA formation after LPS or PA injury. Immunofluorescence images of HA and HC localization in formalin-fixed, paraffin-embedded lung sections from control (0 dpi) and PA-injured (1 and 2 dpi) mice, using antibodies against HA binding protein (red) or HC2 (green), and staining for nuclei with DAPI (Blue). Staining control provided in the last row, using secondary antibody only. Note HC-HA co-localization in the peri-broncho (Br)-vascular (V) interstitium (white arrow); scale bar 50 um. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-018-0812-1), licensed under a CC-BY license.
View 4 more images

Applications for ITIH2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Frozen

Reported in scientific literature (PMID: 29855321)

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

Reported in scientific literature (PMID: 30623115).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ITIH2

The inter-alpha-trypsin inhibitors (ITI) are a family of structurally related plasma serine protease inhibitors involved in extracellular matrix stabilization and in prevention of tumor metastasis. The ITI family contains multiple proteins made up of a light chain (see MIM 176870) and a variable number of heavy chains (Salier et al., 1987 (PubMed 2446322); Himmelfarb et al., 2004 (PubMed 14744536)).(supplied by OMIM)

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 2

Alternate Names

H2P, IGHEP2, Inter-Alpha (Globulin) Inhibitor H2, Intin2, ITI Heavy Chain H2, ITI-H2, ITI-HC2, SHAP

Gene Symbol

ITIH2

UniProt

P19823 (Human)

Additional ITIH2 Products

Product Documents for ITIH2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITIH2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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