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ITIH4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81777

Novus Biologicals, part of Bio-Techne
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NBP1-81777
NBP1-81777-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LAYSFVTPLTSMVVTKPDDQEQSQVAEKPMEGESRNRNVHSAGAAGSRMNFRPGVLSSRQLGLPGPPDVPDHAAYHPFRRLAILPASAPPATSNPDPAVSRVMNMKIEETTMTTQTPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ITIH4 Antibody

Immunohistochemistry-Paraffin: ITIH4 Antibody [NBP1-81777]

Immunohistochemistry-Paraffin: ITIH4 Antibody [NBP1-81777]

Immunohistochemistry-Paraffin: ITIH4 Antibody [NBP1-81777] - Staining in human skeletal muscle and colon tissues using anti-ITIH4 antibody. Corresponding ITIH4 RNA-seq data are presented for the same tissues.
Western Blot: ITIH4 Antibody [NBP1-81777]

Western Blot: ITIH4 Antibody [NBP1-81777]

Western Blot: ITIH4 Antibody [NBP1-81777] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue
Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: ITIH4 Antibody [NBP1-81777]

Immunocytochemistry/ Immunofluorescence: ITIH4 Antibody [NBP1-81777]

Immunocytochemistry/Immunofluorescence: ITIH4 Antibody [NBP1-81777] - Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Applications for ITIH4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ITIH4

Long Name

Inter-alpha-Trypsin Inhibitor Heavy Chain H4

Alternate Names

gp120, H4P, IHRP, ITI-HC4, ITIHL1, PK120

Entrez Gene IDs

3700 (Human)

Gene Symbol

ITIH4

UniProt

Additional ITIH4 Products

Product Documents for ITIH4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ITIH4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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