Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ITK Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human IL2-Inducible T-cell Kinase using the numbering given in entry NP_005537.3 (GeneID 3702).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ITK Antibody

Western Blot: ITK Antibody [NBP2-32240]

Western Blot: ITK Antibody [NBP2-32240]

Western Blot: ITK Antibody [NBP2-32240] - Samples: Whole cell lysate (50 ug) from HeLa (RIPA), 293T (RIPA), and Jurkat (RIPA) cells. Antibodies: Affinity purified rabbit anti-ITK antibody NBP2-32240 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ITK Antibody

Application
Recommended Usage

Western Blot

1:500 - 1:2500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ITK

Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4).

Long Name

IL-2-inducible T-cell Kinase

Alternate Names

EMT, LYK, PSCTK2, Tcsk

Gene Symbol

ITK

UniProt

NP_005537.3 (Human)

Additional ITK Products

Product Documents for ITK Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ITK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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