ITPK1 Antibody (2C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003705-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 2C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ITPK1 Antibodies »

Product Specifications

Immunogen

ITPK1 (AAH03622, 1 a.a. ~ 314 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQTFLKGKRVGYWLSEKKIKKLNFQAFAELCRKRGMEVVQLNLSRPIEEQGPLDVIIHKLTDVILEADQNDSQSLELVHRFQEYIDAHPETIVLDPLPAIRTLLDRSKSYELIRKIEAYMEDDRICSPPFMELTSLCGDDTMRLLEKNGLTFPFICKTRVAHGTNSHEMAIVFNQEGLNAIQPPCVVQNFINHNAVLYKVFVVGESYTVVQRPSLKNFSAGTSDRESIFFNSHNVSKPESSSVLTELDKIEGVFERPSDEVIRELSRALRQALGVSLFGIDIIINNQTGQHAVIDINAFPGDCQVCFIEGWKTD

Specificity

ITPK1 (2C8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ITPK1 Antibody (2C8)

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01]

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01]

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01] - Analysis of ITPK1 expression in transfected 293T cell line by ITPK1 monoclonal antibody (M01), clone 2C8.Lane 1: ITPK1 transfected lysate(35.6 KDa).Lane 2: Non-transfected lysate.
Western Blot: ITPK1 Antibody (2C8) [H00003705-M01]

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01]

Western Blot: ITPK1 Antibody (2C8) [H00003705-M01] - Analysis of ITPK1 over-expressed 293 cell line, cotransfected with ITPK1 Validated Chimera RNAi ( Cat # H00003705-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ITPK1 monoclonal antibody (M01), clone 2C8 (Cat # H00003705-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: ITPK1 Antibody (2C8) [H00003705-M01] - Detection limit for recombinant GST tagged ITPK1 is 0.1 ng/ml as a capture antibody.

Applications for ITPK1 Antibody (2C8)

Application
Recommended Usage

Western Blot

1:500RNAi Validation
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ITPK1

Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetraphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain

Alternate Names

EC 2.7.1.134, EC 2.7.1.159, inositol 13,4-triphosphate 5/6 kinase, inositol 13,4-trisphosphate 5/6 kinase, Inositol 13,4-trisphosphate 5/6-kinase, inositol-tetrakisphosphate 1-kinase, Inositol-triphosphate 5/6-kinase, Ins(13,4)P(3) 5/6-kinase, ITRPK1

Entrez Gene IDs

3705 (Human)

Gene Symbol

ITPK1

OMIM

601838 (Human)

UniProt

AAH03622 (Human)

Additional ITPK1 Products

Product Documents for ITPK1 Antibody (2C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ITPK1 Antibody (2C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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