ITPK1 Antibody (JE64-35)

Western Blot: ITPK1 Antibody (JE64-35) [NBP3-32485]

Western Blot: ITPK1 Antibody (JE64-35) [NBP3-32485] - Western blot analysis of ITPK1 on different lysates with Rabbit anti-ITPK1 antibody (NBP3-32485) at 1/500 dilution.

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: 293T cell lysate
Lane 4: A431 cell lysate
Lane 5: MCF-7 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 46 kDa
Observed band size: 42 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32485) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: ITPK1 Antibody (JE64-35) [NBP3-32485]

Immunohistochemistry: ITPK1 Antibody (JE64-35) [NBP3-32485] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ITPK1 antibody (NBP3-32485) at 1/100 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32485) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.