Jak2 Antibody (6-D3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66913

Novus Biologicals, part of Bio-Techne
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NBP2-66913
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Microarray, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 6-D3

Concentration

2 mg/ml

View all Jak2 Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human Jak2 aa 883-1,132 / 1,132. (SwissProt: O60674 Human)

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Localization

Cytopasm, nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Jak2 Antibody (6-D3)

Western Blot: Jak2 Antibody (6-D3) [NBP2-66913]

Western Blot: Jak2 Antibody (6-D3) [NBP2-66913]

Western Blot: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis on K562 cell lysate using anti-JAK2 mouse mAb.
Immunocytochemistry/ Immunofluorescence: Jak2 Antibody (6-D3) [NBP2-66913]

Immunocytochemistry/ Immunofluorescence: Jak2 Antibody (6-D3) [NBP2-66913]

Immunocytochemistry/Immunofluorescence: Jak2 Antibody (6-D3) [NBP2-66913] - Staining JAK2 in HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100, PBS.
Immunohistochemistry-Paraffin: Jak2 Antibody (6-D3) [NBP2-66913]

Immunohistochemistry-Paraffin: Jak2 Antibody (6-D3) [NBP2-66913]

Immunohistochemistry-Paraffin: Jak2 Antibody (6-D3) [NBP2-66913] - Analysis of paraffin-embedded human breast carcinoma tissue using anti- JAK2 mouse mAb.
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Applications for Jak2 Antibody (6-D3)

Application
Recommended Usage

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry-Paraffin

1:200

Microarray

Validated from a verified customer review

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-66913 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.2% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Jak2

Jak2 is a member of the Janus family tyrosine kinases (Jaks), whose function is to transduce extracellular signals from cytokines and interferons. Jaks associate with cytokine receptors; upon ligand binding, Jaks phosphorylate the receptor. The phosphorylated receptors are bound by SH2-containing proteins, one class of which is the signal transducers and activators of transcription (STATs). Activated STATs, then, translocate to the nucleus to mediate gene transcription (1,2). Tyr1007/1008 of Jak2 are the homologous Tyr1054/1055 in Tyk2, and phosphorylation at these residues is needed for Tyk2 activation (3). A chromosomal aberration involving Jak2 is found in a form of pre-B acute myeloid leukemia (4).

Long Name

Janus Kinase 2

Alternate Names

EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2

Gene Symbol

JAK2

Additional Jak2 Products

Product Documents for Jak2 Antibody (6-D3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Jak2 Antibody (6-D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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