Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67429
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SY24-03
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr1007 and 1008 of human Jak2. (SwissProt: O60674 Human; SwissProt: Q62120 Mouse; SwissProt: Q62689 Rat)
Modification
p Tyr1007, p Tyr1008
Localization
Nucleus, Endomembrane system, Cytoplasm.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03)
Western Blot: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - ICC staining of Phospho-JAK2(Y1007+Y1008) in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor (TM) 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Immunocytochemistry/Immunofluorescence: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Staining Phospho-JAK2(Y1007+Y1008) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03) [NBP2-67429] - Analysis of paraffin-embedded mouse kidney tissue using anti-Jak2 [p Tyr1007, p Tyr1008] antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Applications for Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:200-1:500
Immunohistochemistry-Paraffin
1:200-1:500
Immunoprecipitation
Assay dependent concentration
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 1 using NBP2-67429 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Jak2
Long Name
Janus Kinase 2
Alternate Names
EC 2.7.10, EC 2.7.10.2, JAK-2, Janus kinase 2Janus kinase 2 (a protein tyrosine kinase), JTK10, tyrosine-protein kinase JAK2
Gene Symbol
JAK2
Additional Jak2 Products
Product Documents for Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03)
Product Specific Notices for Jak2 [p Tyr1007, p Tyr1008] Antibody (SY24-03)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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