JAM-C Antibody (CRAM-18F26)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11221

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Product Datasheet / COA / SDS

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Functional, Immunoassay, Immunohistochemistry-Frozen, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # CRAM-18F26

Concentration

0.1 mg/ml

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Product Specifications

Immunogen

The monoclonal antibody CRAM-18 F26 recognizes junctional adhesion molecule-C (JAM-C) also known as JAM-2, a 45 kD cell adhesion molecule (CAM).

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Endotoxin Level

<24 EU/mg

Applications for JAM-C Antibody (CRAM-18F26)

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

For Immunohistochemistry and Flow Cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition of biological activity, dilutions have to be made according to the amounts of JAM-C to be inhibited.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: JAM-C

The monoclonal antibody CRAM-18 F26 recognizes junctional adhesion molecule-C (JAM-C) also known as JAM-2, a 45 kD cell adhesion molecule (CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. Monoclonal antibody CRAM-18 F26 also reacts with human JAM-C. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Long Name

Junctional Adhesion Molecule C

Alternate Names

CD323, JAM3, JAMC

Gene Symbol

JAM3

Additional JAM-C Products

Product Documents for JAM-C Antibody (CRAM-18F26)

Product Datasheets

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COA

SDS

Product Specific Notices for JAM-C Antibody (CRAM-18F26)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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