Jarid1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03856

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Jarid1A Antibodies »

Product Summary for Jarid1A Antibody - BSA Free

Immunogen

Recombinant protein of human Jarid1A.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Jarid1A Antibody - BSA Free

Western Blot: Jarid1A AntibodyBSA Free [NBP3-03856]

Western Blot: Jarid1A AntibodyBSA Free [NBP3-03856]

Western Blot: Jarid1A Antibody [NBP3-03856] - Analysis of extracts of various cell lines, using Jarid1A antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.
Chromatin Immunoprecipitation: Jarid1A Antibody [NBP3-03856] - Chromatin immunoprecipitation analysis of extracts of HCT116 cells, using Jarid1A antibody (NBP3-03856) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunoprecipitation: Jarid1A Antibody - BSA Free [NBP3-03856]

Immunoprecipitation: Jarid1A Antibody - BSA Free [NBP3-03856]

Immunoprecipitation: Jarid1A Antibody [NBP3-03856] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug Jarid1A antibody (NBP3-03856). Western blot was performed from the immunoprecipitate using Jarid1A antibody (NBP3-03856) at a dilution of 1:500.

Applications for Jarid1A Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100 - 1:500

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Jarid1A

Jarid1A is encoded by this gene is a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein which regulates cell proliferation. This protein also interacts with rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Rhombotin-2 is thought to either directly affect the activity of the encoded protein or may indirectly modulate the functions of the retinoblastoma protein by binding to this protein. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Long Name

Lysine-specific demethylase 5A

Alternate Names

EC 1.14.11.67, KDM5A, RBBP-2, RBBP2, RBP2

Gene Symbol

KDM5A

Additional Jarid1A Products

Product Documents for Jarid1A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Jarid1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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