JAZF1 Antibody (PCRP-JAZF1-1C2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13885

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-JAZF1-1C2

Concentration

0.2 mg/ml

View all JAZF1 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary) (Uniprot: Q86VZ6)

Reactivity Notes

Predicted to react in Mouse.

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

27.08 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14221)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for JAZF1 Antibody (PCRP-JAZF1-1C2)

Immunocytochemistry/ Immunofluorescence: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885]

Immunocytochemistry/ Immunofluorescence: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885]

Immunocytochemistry/Immunofluorescence: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Immunofluorescence analysis of PFA-fixed HeLa cells. JAZF1 Antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885]

Immunohistochemistry-Paraffin: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885]

Immunohistochemistry-Paraffin: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Formalin-fixed, paraffin-embedded human testis stained. Strong nuclear staining using JAZF1 antibody (PCRP-JAZF1-1C2) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885]

Flow Cytometry: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885]

Flow Cytometry: JAZF1 Antibody (PCRP-JAZF1-1C2) [NBP3-13885] - Flow cytometric analysis of PFA-fixed HeLa cells. JAZF1 antibody (PCRP-JAZF1-1C2) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).
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Applications for JAZF1 Antibody (PCRP-JAZF1-1C2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: JAZF1

The JAZF1 gene encodes a nuclear protein with three C2H2-type zinc fingers, and functions as a transcriptional repressor.Chromosomal aberrations involving this gene are associated with endometrial stromal tumors. Alternatively splicedvariants which encode diff

Alternate Names

JAZF zinc finger 1, juxtaposed with another zinc finger gene 1, juxtaposed with another zinc finger protein 1, TAK1-interacting protein 27, TIP27ZNF802DKFZp761K2222, Zinc finger protein 802

Gene Symbol

JAZF1

UniProt

Q86VZ6 (Human)

Additional JAZF1 Products

Product Documents for JAZF1 Antibody (PCRP-JAZF1-1C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JAZF1 Antibody (PCRP-JAZF1-1C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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