JMJD2B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74604
![Immunoprecipitation: JMJD2B Antibody [NB100-74604]](https://resources.bio-techne.com/images/products/JMJD2B-Antibody-NB100-74604-img0003.jpg "Immunoprecipitation: JMJD2B Antibody [NB100-74604]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Chicken
Cited:
Avian - Chicken
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, Western Blot
Cited:
Chemotaxis, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).
Reactivity Notes
Chicken reactivity reported in scientific literature (PMID: 26598618)
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for JMJD2B Antibody
Immunoprecipitation: JMJD2B Antibody [NB100-74604]
Immunoprecipitation: JMJD2B Antibody [NB100-74604] - Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JMJD2B antibody NB100-74604 used for IP at 3 ug per reaction. JMJD2B was also immunoprecipitated by rabbit anti-JMJD2B antibody NB100-74605. For blotting immunoprecipitated JMJD2B, NB100-74604 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for JMJD2B Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Use in Western blot, and chromatin immunoprecipitation reported in scientific literature (PMID 26598618).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: JMJD2B
Long Name
Lysine-specific demethylase 4B
Alternate Names
EC 1.14.11.66, JHDM3B, KDM4B, KIAA0876
Entrez Gene IDs
23030 (Human)
Gene Symbol
KDM4B
UniProt
Additional JMJD2B Products
Product Documents for JMJD2B Antibody
Product Specific Notices for JMJD2B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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