JNK3 Antibody (10E4A4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51544

Novus Biologicals, part of Bio-Techne
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NBP1-51544
NBP1-51544-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10E4A4

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all JNK3 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human JNK3 (aa28-233) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for JNK3 Antibody (10E4A4) - BSA Free

Western Blot: JNK3 Antibody (10E4A4)BSA Free [NBP1-51544]

Western Blot: JNK3 Antibody (10E4A4)BSA Free [NBP1-51544]

Western Blot: JNK3 Antibody (10E4A4) [NBP1-51544] - Analysis using MAPK10 mouse mAb against NIH/3T3 (1) and SKN-SH (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: JNK3 Antibody (10E4A4) - BSA Free [NBP1-51544]

Immunocytochemistry/ Immunofluorescence: JNK3 Antibody (10E4A4) - BSA Free [NBP1-51544]

Immunocytochemistry/Immunofluorescence: JNK3 Antibody (10E4A4) [NBP1-51544] - Immunofluorescence staining of methanol-fixed A431 (left) and Hela (right) cells showing cytoplasmic and membrane localization.

Applications for JNK3 Antibody (10E4A4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JNK3

C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3).

Long Name

C-Jun N-terminal Kinase 3

Alternate Names

MAPK10, SAPK1 beta

Gene Symbol

MAPK10

Additional JNK3 Products

Product Documents for JNK3 Antibody (10E4A4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JNK3 Antibody (10E4A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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