JPH2 Antibody (3-2-R)

Western Blot: JPH2 Antibody (3-2-R) [NBP3-32488]

Western Blot: JPH2 Antibody (3-2-R) [NBP3-32488] - Western blot analysis of JPH2 on rat skeletal muscle tissue lysates with Mouse anti-JPH2 antibody (NBP3-32488) at 1/1,000 dilution.

Lysates/proteins at 20 ug/Lane.

Predicted band size: 74 kDa
Observed band size: 70 kDa

Exposure time: 3 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32488) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: JPH2 Antibody (3-2-R) [NBP3-32488]

Immunohistochemistry: JPH2 Antibody (3-2-R) [NBP3-32488] - Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Mouse anti-JPH2 antibody (NBP3-32488) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32488) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.