JunB/AP-1 Antibody (3U10K1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16491

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16491-100ul
NBP3-16491-20ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3U10K1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all JunB Antibodies »

Product Summary for JunB/AP-1 Antibody (3U10K1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human JunB/AP-1 (P17275). MCTKMEQPFYHDDSYTATGYGRAPGGLSLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTGASLKLASSELERLIVPNSNGVIT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunB/AP-1 Antibody (3U10K1)

Western Blot: JunB/AP-1 Antibody (3U10K1) [NBP3-16491]

Western Blot: JunB/AP-1 Antibody (3U10K1) [NBP3-16491]

Western Blot: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Western blot analysis of extracts of BxPC-3 cells, using JunB/AP-1 Rabbit mAb (NBP3-16491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (3U10K1) [NBP3-16491]

Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (3U10K1) [NBP3-16491]

Immunohistochemistry-Paraffin: JunB/AP-1 Antibody (3U10K1) [NBP3-16491] - Immunohistochemistry of paraffin-embedded human esophageal using JunB/AP-1 Rabbit mAb (NBP3-16491) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for JunB/AP-1 Antibody (3U10K1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: JunB

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Long Name

JunB Proto-oncogene

Alternate Names

activator protein 1, AP-1, jun B proto-oncogene, transcription factor jun-B

Gene Symbol

JUNB

Additional JunB Products

Product Documents for JunB/AP-1 Antibody (3U10K1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for JunB/AP-1 Antibody (3U10K1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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