JunB/AP-1 Antibody (PCRP-JUNB-3G11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07578

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2C Clone # PCRP-JUNB-3G11

Concentration

0.2 mg/ml

View all JunB Antibodies »

Product Summary for JunB/AP-1 Antibody (PCRP-JUNB-3G11)

Immunogen

Recombinant full-length human JunB/AP-1 protein (Uniprot: P17275)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2C

Theoretical MW

35.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08390)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for JunB/AP-1 Antibody (PCRP-JUNB-3G11)

Immunocytochemistry/ Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578]

Immunocytochemistry/ Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578]

Immunocytochemistry/Immunofluorescence: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Immunofluorescence Analysis of HeLa cells using JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JUNB-3G11) followed by goat anti- Mouse IgG-CF488 (green). Phalloidin counterstain.
Flow Cytometry: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578]

Flow Cytometry: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578]

Flow Cytometry: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Flow Cytometric Analysis of PFA-fixed HeLa cells. JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JUNB-3G11) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578]

Protein Array: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578]

Protein Array: JunB/AP-1 Antibody (PCRP-JUNB-3G11) [NBP3-07578] - Analysis of Protein Array containing more than 19,000 full-length human proteins using JunB/AP-1 Mouse Monoclonal Antibody (PCRP-JunB/AP-1-3G11).

Applications for JunB/AP-1 Antibody (PCRP-JUNB-3G11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: JunB

The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.

Long Name

JunB Proto-oncogene

Alternate Names

activator protein 1, AP-1, jun B proto-oncogene, transcription factor jun-B

Gene Symbol

JUNB

UniProt

P17275

Additional JunB Products

Product Documents for JunB/AP-1 Antibody (PCRP-JUNB-3G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JunB/AP-1 Antibody (PCRP-JUNB-3G11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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