JunD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24931

Novus Biologicals, part of Bio-Techne
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NBP3-24931
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all JunD Antibodies »

Product Summary for JunD Antibody

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein JunD using the following amino acid sequence: AAGSMMKKDALTLSLSEQVAAALKPA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for JunD Antibody

JunD Antibody Immunocytochemistry/Immunofluorescence: JunD Antibody [NBP3-24931]

Immunocytochemistry/Immunofluorescence: JunD Antibody [NBP3-24931]

Staining of human cell line MCF-7 shows localization to nucleoplasm.
JunD Antibody Western Blot: JunD Antibody [NBP3-24931]

Western Blot: JunD Antibody [NBP3-24931]

Analysis in human cell line RT-4 and human cell line U-251 MG.

Applications for JunD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: JunD

The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

Long Name

JunD Proto-oncogene

Alternate Names

AP-1

Gene Symbol

JUND

Additional JunD Products

Product Documents for JunD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for JunD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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